RBRC05324
C (3-6 months)
Developed by Michael McBurney, Ottawa Regional Cancer Centre and Department of Medicine, University of Ottawa. A knock-in vector carrying a point mutation in exon 5 of Sirt1 gene, in which codon 355 was mutated from CAT (histidine) to TAT (tyrosine) was inserted into exon 5. A hygromycin resistant cassette flanked by loxP sites was removed by crossing with Prm-Cre mice (129-Tg (Prm-Cre) 580g / J).
<a href='https://brc.riken.jp/mus/pcr05324'>Genotyping protocol -PCR-</a>
Cre/loxP system
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Michael W. McBurney
Michael W. McBurney
条件を付加する。<br>In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Heterozygote X Wild-type
SirT1 variant (H355Y) knock-in mice, carrying a point mutation in exon 5 of Sirt1 gene, in which codon 355 was mutated from CAT (histidine) to TAT (tyrosine). Homozygous mutant mice are infertile.
129-Sirt1<tm2.1Mcby>
129-Sirt1<tm2.1Mcby>
Phage P1 loxP site, mouse Sirt1 genomic DNA
Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol>
true
R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
C(3〜6か月)
Sirt1 KI/H355Y, STOCK Sirt1<tm2.1Mcby>
Sirt1 KI/H355Y, STOCK Sirt1<tm2.1Mcby>